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Molecule | Mesenchyme |Basement Membrane| Epithelium |Species| Gland |Stage| Expt. Methd. | Remarks | Reference
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| | | | | | | | |
| | | | | | | | |
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BM-1 | ++ close to |+++ lobules and | 0? | Mouse |Submand|E13+ |Immunofluor. |No expression at |Hardman,P. 1992
| lobules only. |adjacent ducts. | | |Subling|48hrs|Organ culture. |base of ducts. |
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cea10 | Expessed within gland,product MAY be | Mouse |Not |Adult|RNase |putative cell adh.|Keck,U. 1995
| developmentally expressed | |stated | |protection |molecule. |
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Clusterin | 0 | / | 0 | Mouse |Not |E14.5|In-situ |Expressed in lung,|French,LE. 1993
(mRNA)| | | | |stated |E18.5| |pancreas, kidney. |
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Collagen |+++ extracellular | ++ uniform | 0 | Mouse |Submand|E12 &|Immunohist. | |Nakanishi,Y. 1988
type-I | matrix. | distribution. | | | | E13 | | |
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Collagen |+++ concentrated | +/0 | 0 | Mouse |Submand|E13 +|Immunofluor. |Distribution more |Hardman,P. 1992
type-I | deep in clefts. | | | |Subling|72hrs|Organ culture. |diffuse at 24hrs. |
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Collagen |+++ in the stroma | + | 0 | Rat |Parotid|0-14d|Northern&immuno|Falls to adult |Lazowski,K.W. 1994
alpha1(I) |of the mesenchyme | | | | | pp |tissue sect. |levels by day 21 |
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Collagen |+++ extracellular |++ discontinuous | 0 | Mouse |Submand| E12 |Immunohist. | |Nakanishi,Y. 1988
type-III | matrix. | | | | |early| | |
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Collagen |+++ concentrated |++ more uniform | 0 | Mouse |Submand| E13 |Immunohist. |Stabilises clefts?|Nakanishi,Y. 1988
type-III | in clefts. | distribution. | | | |late | | |
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Collagen | Capilary | +++ uniform | 0 | Mouse |Submand|E12 &|Immunohist. | |Nakanishi,Y. 1988
type-IV | endothelium. | distribution. | | | | E13 | | |
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Collagen | ++ |+++ lobules and | 0? | Mouse |Submand|E13+ |Immunofluor. |Only around |Hardman,P. 1992
type-IV | |ducts at 24hrs. | | |Subling|24&48|Organ culture. |lobules at 48hrs. |
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Collagen | ++ | +++ around | 0? | Rat |Submand|E16 |Immunohist. |Rapidly growing |Kadoya,Y 1989
type-IV | | term. clusters. | | | |toE18|tissue sections|front |
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Collagen | ? |+++ developing | 0 | Rat |Parotid|0-14d|Northern&immuno|Falls to adult |Lazowski,K.W. 1994
alpha1(IV)| |acini and ducts. | | | | pp |tissue sections|levels by day 21 |
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Collagen | +++ extracellular| +++ uniform | 0 | Mouse |Submand|E12 &|Immunohist. | |Nakanishi,Y. 1988
type-V | matrix. | distribution. | | | | E13 | | |
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Collagen | +++ adjacent to |+++ surrounding | 0? | Mouse |Submand|E13+ |Immunofluor. | |Hardman,P. 1992
type-V | lobes and ducts. |lobes and ducts. | | |Subling|24&48|Organ culture. | |
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cra | Expressed in centre of cell clusters | Human |Parotid|Adult|Immunohist |Disrupts intercel-|Iwai,M. 1991
| in dissociated gland cultures. | |Submand| | |lular adhesion? |
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Epimorphin| + | 0 | 0 | Mouse |Submand|13day|Immunofluor. to| |Kadoya,Y 1995
| | | | | | |tissue sections| |
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Epimorphin| +++ | 0 | 0 | Mouse |Submand|17day|Immunofluor. to| |Kadoya,Y 1995
| | | | | | |tissue sections| |
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Fibro- | +++ concentrated | 0 | 0 | Mouse |Submand|E13to|Immunofluor. | No change in |Hardman,P. 1992
nectin | between lobules | | | |Subling|72hrs|Organ culture. |distrib with time.|
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Glycosami-|Rate of turnover is higher at the distal ends of | Mouse |Submand|E13.5|Biochemical |Balance between |Bernfield,M.R. 1972
-noglycans|lobules (disrupted BM) than in the clefts. | | | |Histochemical |synth.& degrad. |Bernfield,M.R. 1982
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Glycosami-|In the basal lamina, consists of 50% hyaluronic acid, | Mouse |Submand| E13 |Biochemical | |Cohn,R.H. 1977
-noglycans|40% chondroitin sulphate. (ratio ch-4-s:ch-6-s, 2:1) | | | |assay. | |
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Glycosami-|Relative proportions of sulphated glycosaminoglycan in | Rat |Submand| E17 |Biochemical | |Cutler,L.S. 1991
-noglycans|acinii. Chondroitin sulphate-73%, Heparan sulphate-26% | | | |assay. | |
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Glycosami-|Relative proportions of sulphated glycosaminoglycan in | Rat |Submand| E18 |Biochemical |Rapid change at |Cutler,L.S. 1991
-noglycans|acinii. Chondroitin sulphate-41%, Heparan sulphate-54% | | | |assay. |cytodifferentiat. |
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Glycosami-|Relative proportions of sulphated glycosaminoglycan in | Rat |Submand|E18- |Biochemical |Progressive change|Cutler,L.S. 1991
-noglycans|acinii. Chondroitin sulphate-10%, Heparan sulphate-85% | | |-P35 |assay. |to adult levels. |
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Integrin | Not localised, quantified. | Rat |Parotid|Adult|ELISA |Vascular endothel,|Voigt,S. 1994
alpha-1 | Parotid gland 500ng/mg, submandibular gland 270ng/mg | |Submand| | |& smooth muscle. |
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Integrin | ? | / | +++ basally and | Human |Parotid|Adult|Immunohist, | |Franchi,A. 1994
alpha-2 | | |cell-cell contacts| | | |tissue sections| |
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Integrin | ++ spindle shaped| / | 0 | Human |Parotid|Adult|Immunohist, |Vascular |Franchi,A. 1994
alpha-5 | cells. | | | | | |tissue sections|endothelium. |
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Integrin | 0 | / |+++ esp. basal to | Mouse |Submand|13day|Immunofluor. to|Stains capillary |Kadoya,Y 1995
alpha-6 | | |peripheral cells | | | |tissue sections|endothelium. |
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Integrin | 0 | / |++ surface of all | Mouse |Submand|E13.5|Immunoelectron.|Slightly stronger |Kadoya,Y 1993
alpha-6 | | | epith. cells | | | |tissue sections|towards B.M. |
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Integrin | 0 | / |++ peripheral | Mouse |Submand| E15 |Imunoelectron. |No expression in |Kadoya,Y 1993
alpha-6 | | |epith. cells only.| | | |tissue sections|more central cells|
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Integrin | 0 | / |++basolat.surface,| Mouse |Submand|E17- |Immunofluor. |Signal stronger in|Kadoya,Y 1993
alpha-6 | | |acinotubular cells| | |birth|Immunoelectron |acini. |Kadoya,Y 1995
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Integrin | +++ | Rat |Parotid|0-14d|Northern. |Undetectable after|Lazowski,K.W. 1994
alpha-6 | Northern only, not localised | | | pp | |day 21. |
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Integrin | +++ surrounding | / | +++ | Mouse |Submand|13day|Immunofluor. to| |Kadoya,Y 1995
beta-1 | the epithelium. | | | | |17day|tissue sections| |
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Integrin | 0 | / | +++ developing | Rat |Parotid|0-14d|Northern&immuno|Detectable levels |Lazowski,K.W. 1994
beta-1 | | | acini and ducts. | | | pp |tissue sections|(++) after day21. |
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Integrin | 0 | / |+ basal surface of| Mouse |Submand|13day|Immunofluor. to| |Kadoya,Y 1995
beta-4 | | |cells next to BM. | | | |tissue sections| |
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Integrin | 0 | / | ++ | Mouse |Submand|17day|Immunofluor. to| |Kadoya,Y 1995
beta-4 | | | | | | |tissue sections| |
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Laminin-1 |++ in the extra- | +++ | 0 | Mouse |Submand|13day|Immunofluor. to|Capilliaries stain|Kadoya,Y 1995
|cellular matrix | | | | | |tissue sections|Polyclonal ab. |
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Laminin | +/0 |+++ around term.| + cytoplasm | Mouse |Submand|E13.5|Immunohist, | |Kadoya,Y 1993
| | clusters. |terminal clusters.| | | |tissue sections| |
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Laminin | +/0 |+++ around term.| 0 | Mouse |Submand| E14 |Immunohist, |Term. cluster, |Kadoya,Y 1993
| | clusters. | | | | |tissue sections|acinotube & stalk |
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Laminin | 0 |+++ around term.|+++ in ER of cells| Rat |Submand|E15 |Immunohist, |Rapid synthesis |Kadoya,Y 1989
| | clusters. |at expanding front| | |toE17|tissue sections|during expansion. |
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Laminin-1 | 0 | +++ | 0 | Mouse |Submand|17day|Immunofluor. to|Ab detects alpha-1|Kadoya,Y 1995
| | | | | | |tissue sections|beta-1, gamma-1. |
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Laminin | + |+++ Epith-mesench| 0? | Mouse |Submand|E13+ |Immunofluor. |Fibril-like tracts|Hardman,P. 1992
| | interface | | |Subling|24&48|Organ culture. |in mesenchyme. |
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Laminin-1 | ? |+++ dev. and diff| 0 | Rat |Parotid|0-14d|Northern&immuno|Falls to adult |Lazowski,K.W. 1994
| |acini and ducts. | | | | pp |tissue sections|levels by day21. |
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Laminin | + in the extra- | +++ | 0 | Mouse |Submand|13day|Immunofluor. to|No staining in BM |Kadoya,Y. 1995
alpha-1 |cellular matrix | | | | | |tissue sections|of capilliaries. |
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Laminin |++ cells adjacent | na |+ basal cells at | Mouse |Submand|13day|ISH to tissue |Rudimentary |Kadoya,Y. 1995
alpha-1 | to epith stalk. | |tip of epith stalk| | | |sections |branches only. |
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Laminin | 0 | + around duct | 0 | Mouse |Submand|17day|Immunofluor. to| |Kadoya,Y. 1995
alpha-1 | | epith only. | | | | |tissue sections| |
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Laminin | 0 | na | 0 | Mouse |Submand|17day|ISH to tissue | |Kadoya,Y. 1995
alpha-1 | | | | | | |sections | |
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Laminin | ? | / | +++ | Mouse |Submand|E13.5|In-situ | |Galliano,MF. 1995
alpha-3A | | | | | |E17.5| | |
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Laminin | ? | / | +++ | Mouse |Submand|E13.5|In-situ | |Galliano,MF. 1995
alpha-3B | | | | | |E17.5| | |
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Tenascin-C| 0 | 0 | 0 | Mouse |Submand|13Day|Immunofluor. to| |Kadoya,Y. 1995
| | | | | | |tissue sections| |
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Tenascin-C|+ surrounding duct| 0 | 0 | Mouse |Submand|17Day|Immunofluor. to| |Kadoya,Y. 1995
|epithelium only. | | | | | |tissue sections| |
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Date of last revision: 09 June 1997 - 15:13:06
dh5@holyrood.ed.ac.uk