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Cell Line| Source | Description | Repository/Owner
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A253 |Human submaxilliary gland epidermal |Neoplastic salivary gland cell line. |Giard et al.
|adenocarcinoma. | |ATCC
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HMS-1 |Human parotid gland basal cell |Myoepithelial cell line, epithelial |Sternlicht,MD. 1996 Dept of Pathology
|adenocarcinoma. |morphogenesis with HMS-1 as feeder |University of California, Los Angeles.
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HPAM 1 |Normal human parotid gland. |Untransformed normal diploid epithelial|Chopra,D.P. 1995
HPAF 1 | |acinar cell lines. Continuous culture |
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HSG |Irradiated, histologically normal |Intercalated duct cells. |Shirasuna,K. Tokushima Univ.
|human submandibular gland. |(neoplastic) |Japan (citing ref. Wu,A.J. 1996)
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HSG |HSG cell line briefly treated with |Irreversible differentiation to |Azuma,M. 1986
(treated)|sodium butyrate. |myoepithelial cell phenotype. |
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HSG |HSG cell line briefly treated with |Irreversible differentiation to acinar |Sato,M. (1987)
(treated)|5-azacytidine. |and myoepithelial cell phenotype. |
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HSG |HSG cell line grown in the presence |Induces a reversible phenotypic change |Azuma,M. (1988)
(treated)|of retinoic acid. |to keratinizing squamous cells. |
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HSG |Induced HSG acinar cells grown in the|Induces a reversible phenotypic change |Azuma,M. (1989)
(treated)|presence of dihydroxyvitamin-D3. |to chondrocyte like cells. |
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NS-SV-AC |SV40 immortalised normal human |Acinar cell phenotype. |Azuma,M. 1993. Tokushima Univ.
|salivary gland. | |School of Dentistry, Japan.
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NS-SV-DC |SV40 immortalised normal human |Ductal (cuboidal) phenotype. Increased |Azuma,M. 1993. Tokushima Univ.
|salivary gland. |proteolytic enzyme activity. Matrigel |School of Dentistry, Japan.
| |to induce diff. req. inhibitors Azuma |
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NS-SV-MC |SV40 immortalised normal human |Myoepithelial phenotype. |Azuma,M. 1993. Tokushima Univ.
|salivary gland. | |School of Dentistry, Japan.
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NS-SV-SC |SV40 immortalised normal human |Squamous cell phenotype. |Azuma,M. 1993. Tokushima Univ.
|salivary gland. | |School of Dentistry, Japan.
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PA 16/23 |Pleiomorphic adenoma of the parotid |Myoepithelial cells secreting IL6 |Gallo,O. 1992 Institute of
|gland (human). | |Otolaryngology, Florence. Italy.
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SGT-1 |Human submandibular gland, poorly |Human salivary gland adenocarcinoma. |Dr C.C.Lee, Dangkok Univ. Seoul
|differentiated adenocarcinoma. | |Korea. (ref. Shrestha)
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_______________________________________________________________________________________________________________________ Technique | Description | Details | Ref ======================================================================================================================= Cell |Growth of tissue/explants on chicken |Separation of cell types to establish |Prasad,K.N. 1992 separation |plasma clots. |monocultures and for transfection expts| ----------------------------------------------------------------------------------------------------------------------- Normal |Conditions for continuous culture |F12/DME 3:1, 2.5%FBS, 1mM Ca2+, cholera|Sabatini,L.M. 1991 human cells|using a 3T3 fibroblast feeder layer. |toxin, hydrocortisone, insulin, EGF. | ----------------------------------------------------------------------------------------------------------------------- Ligand-IgG |Ligand/reporter hybrid protein made |Ligand binds specifically to receptor, |LaRochelle,W.J. 1995 chimera |using a mammalian expression vector. |reporter (IgG) is detected immunohist. | -----------------------------------------------------------------------------------------------------------------------
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Antibody | Source | Use | Repository/Owner
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50B8 |Rat Monoclonal CH3 mouse mammary |170kD Glycoprotein. Specific for proacinar |A Vanleeuwenhoek, (ref. Sonnenberg)
|tumour crude membrane prep. |and mature acinar cells. (ref. Durban) |Netherlands Cancer Inst. Amsterdam.
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33A10 |Rat Monoclonal CH3 mouse mammary |Glycoprotein. Specific for differentiating |A Vanleeuwenhoek, (ref. Sonnenberg)
|tumour crude membrane prep. |acinar and ductal cells. (ref. Durban) |Netherlands Cancer Inst. Amsterdam.
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50B8 |Rat Monoclonal CH3 mouse mammary |Glycoprotein. Specific for striated duct |A Vanleeuwenhoek, (ref. Sonnenberg)
|tumour crude membrane prep. |cells and GCT cells. (ref. Durban) |Netherlands Cancer Inst. Amsterdam.
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CY15B8 |Mouse immunized with collagen XIV|Specific for NC3 domain of collagen (XIV), |Lethias,C. 1993
|prep from bovine skin. |study of epith/mesenchyme interactions? |CNRS. UPR, 412. Lyon. France.
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Ham8 |Crude gap junction fractions from|Recognises salivary gland acinar cell gap |Dr Fujikura,
|rat liver. |junctions but not ductal cells (connexin 32)|Yamagucci Univ. Japan.
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Last update: 24 October 1996 - 15:25:53
dh5@holyrood.ed.ac.uk